Process HPLC Column, RP C4, Wide Pore, 5um, 300A. 50mm ID x 50mm Length. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP C4™ Wide Pore is a reversed-phase HPLC stationary phase designed for process-scale purification of proteins, peptides, enzymes, and other large hydrophobic or neutral compounds. Manufactured using a four-carbon butyl ligand bonded to ultra-high purity silica and fully double end capped, this 300 Å wide pore stationary phase provides enhanced accessibility for large biomolecules while maintaining the reduced hydrophobic retention characteristics of C4 chemistry. The wide pore design is particularly beneficial for large-molecule purification where recovery, loading capacity, and process efficiency are critical.
68504-P50-50-3M is a process HPLC column packed with 5 µm RP C4™ Wide Pore particles featuring a 300 Å pore size in a 50 mm ID x 50 mm format. The large 50 mm internal diameter provides substantial loading capacity for production and process-scale purification, while the shorter 50 mm length is optimized for rapid cycle times and high-throughput operation. The 300 Å pore structure improves accessibility and mass transfer for proteins, peptides, enzymes, and other large biomolecules. Typical operating flow rates are approximately 80–150 mL/min depending on analyte characteristics, mobile phase composition, and system capabilities.
This column is particularly useful for process purification of proteins, peptides, enzymes, and other large biomolecules in pharmaceutical, biopharmaceutical, and manufacturing environments. Compared to more hydrophobic C8 and C18 phases, the C4 bonded phase generally provides lower retention, which can improve recovery and purification efficiency for larger analytes. Due to its size and flow requirements, a specialized process HPLC system is required for operation.
Key Benefits
RP C4™ Wide Pore stationary phase
USP L26 equivalent chemistry
Butyl (C4) bonded phase
Fully double end capped
Ultra-high purity silica support
300 Å wide pore design
High loading capacity
Optimized for proteins and peptides
Supports high flow rates
Ideal for high-throughput process-scale bioseparations
Typical Applications
Process HPLC
Protein purification
Peptide purification
Enzyme purification
Biomolecule purification
Biopharmaceutical manufacturing
Process-scale chromatography
Fraction collection
Process development
Production-scale purification
Specifications
Product Type: Process HPLC Column
Stationary Phase: RP C4™ Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 50 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 80–150 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Designed for process-scale purification of proteins, peptides, enzymes, and other large biomolecules
300 Å wide pores improve accessibility for larger analytes
Provides less retention than C8 and C18 stationary phases
High loading capacity for manufacturing and production-scale applications
The 50 mm ID format requires a specialized process HPLC system
Typical operating flow rates are approximately 80–150 mL/min
5 µm particles provide robust performance with manageable backpressure
Fully double end capped to minimize secondary interactions
The 50 mm length is ideal for rapid purification cycles and high-throughput processing
Excellent choice for process-scale protein and peptide purification
This is a Special Order Item